bis tris gels (Thermo Fisher)

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Thermo Fisher bis tris gels
Bis Tris Gels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bis+tris+gel/Bis-Tris%2C+%E2%89%A598%25+(Dry+Basis)%2C+Ultrapure/pm42097025-310-7-9
Average 97 stars, based on 1 article reviews

bis tris gels - by Bioz Stars, 2026-10

97/100 stars

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Membrane:

Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

Article Title: Single kinetochores execute an ordered series of molecular events as the spindle assembly checkpoint is silenced.
Article Snippet: Lysates were then clarified by centrifugation for 20 min at 14,000 × g and 4◦C, and quantified using Bradford Reagent (Bio-Rad Laboratories) according to the manufacturer’s instructions. .. 50 μg of cell lysates were separated through SDS-PAGE on a NuPAGE 4-12% Bis-Tris gel (Invitrogen) and transferred to an Immobilon-FL polyvinylidene fluoride membrane (IPFL00010, Millipore). ..

Article Title: PrP C -facilitated cell signaling activates phospholipase Cɣ1 and triggers an Arc/Arg3.1 response in mouse and iPSC-derived human neurons.
Article Snippet: .. Equal amounts of protein were reduced with DTT, electrophoresed through a 10% Bis-Tris gel (Invitrogen), and transferred to a nitrocellulose membrane by wet blotting. ..

Article Title: Thyroid hormone acts independently of the thyroid hormone receptor beta in hepatocytes to improve systemic insulin sensitivity
Article Snippet: .. Protein lysates were resolved in 4%–12% Bis-Tris gel (Invitrogen) and transferred to a nitrocellulose membrane. .. Blots were probed using rabbit polyclonal anti-p-Akt Ser473 antibody (Cell Signaling Technology #9271, 1:2500) or rabbit polyclonal anti-Akt (Cell Signaling Technology #9272, 1:2500) overnight at 4°C with mouse monoclonal anti-beta-actin antibody ( MA191399 ; Fisher Scientific; 1:5000).

Article Title: Inhibition of CDK9 sensitizes multidrug resistant ovarian cancer cells to paclitaxel
Article Snippet: Protein concentrations were calculated by the Protein Assay Reagents (Bio-Rad, Hercules, CA, USA) and a SpectraMax 340PC Microplate Reader from Molecular Devices (San Jose, CA, USA). .. Equal amounts of proteins were separated by NuPAGE ® 4–12% Bis-Tris Gel (Thermo Fisher Scientific) and subsequently transferred to a nitrocellulose membrane (Bio-Rad), which was then incubated with specific primary antibodies at 4 °C overnight: CDK9 (Cell Signaling Technology, Cat.#2316, 1:1000 dilution); RNAPII (Abcam, Cat.#ab817, 1:1000 dilution), S2 RNAPII (Abcam, Cat.#ab5095, 1:1000 dilution); Pgp (Cell Signaling Technology, Cat.#9532, 1:1000 dilution); PARP (Cell Signaling Technology, Cat.#13342, 1:1000 dilution); Stat3 (Cell Signaling Technology, Cat.#9139, 1:1000 dilution); Phospho-Stat3 (Cell Signaling Technology, Cat.#9145, 1:1000 dilution); Mcl-1 (Cell Signaling Technology, Cat.#39224, 1:1000 dilution); Bax (Cell Signaling Technology, Cat.#2772, 1:1000 dilution); α -Tubulin (Cell Signaling Technology, Cat.#3873, 1:1000 dilution). .. After being washed three times for 5 min with TBST, the membranes were further incubated with Goat anti-rabbit IRDye 800CW or Goat anti-mouse IRDye 680LT secondary antibody (LI-COR Biosciences, Lincoln, NE, USA), and scanned by Odyssey ® CLx equipment (LI-COR Biosciences) to detect the bands.

Nucleic Acid Electrophoresis:

Article Title: Abi3 S212F Alzheimer's disease variant alters plaque structure and disrupts microglia
Article Snippet: Protein concentrations were determined by the Pierce protein assay (22660; Thermofisher Scientific). .. Proteins were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) through a 4%–12% Bis/Tris gel (Life Technologies) for 1.5 h at 125 V. Proteins were transferred to 0.2 μM nitrocellulose membranes (1704158; Bio‐Rad) and stained with REVERT 700 total protein stain (TPS; 926‐11016, LI‐COR) for 5 min at room temperature. ..

Staining:

SDS Page:

Incubation:

other:

Article Title: Adaptation of α-synuclein fibrils following multiple system atrophy transmission to mice
Article Snippet: For TL digestion, samples were digested with 50 μg/mL TL (or same volume of buffer) in a thermomixer (Eppendorf) at 37 °C at 600 rpm for 1 h. The digestion was halted by adding 0.25 M EDTA (Invitrogen) prior to centrifugation at 100,000 x g at 4 °C for 1 h. The supernatant was removed and pellets were resuspended in the same sample buffer.

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